Recombinant Human IL-5

Alternative Names:B-cell differentiation factor I Protein, Colony stimulating factor Protein, EDF Protein, Eosinophil differentiation factor Protein, IL-5 Protein, IL5 Protein, IL5_HUMAN Protein, Interleukin 5 Protein, Interleukin 5 (colony stimulating factor Protein, eosinophil) Protein, Interleukin-5 Protein, T Cell Replacing Factor Protein, T-cell replacing factor Protein, TRF Protein
Catalogue No.:P4506Uniprot ID:P05113
Host:E.ColiExpression Region:20-134
Mol. Weight:15 kDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Human IL-5
Catalogue No.
P4506
Species
Human
Host
E.Coli
Expression Region
20-134
Mol. Weight
15 kDa
Tags
N-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.4-1 ng/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
B-cell differentiation factor I Protein, Colony stimulating factor Protein, EDF Protein, Eosinophil differentiation factor Protein, IL-5 Protein, IL5 Protein, IL5_HUMAN Protein, Interleukin 5 Protein, Interleukin 5 (colony stimulating factor Protein, eosinophil) Protein, Interleukin-5 Protein, T Cell Replacing Factor Protein, T-cell replacing factor Protein, TRF Protein
SDS-PAGE